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6.        Dry the films in shade by waving them in the air.
7.        In the case of films to be examined for protozoa or
blood changes, immerse the slides in acetone-free methyl
alcohol for about 10 minutes and then allow them to dry.
Smears to be examined for bacteria should be fixed by
repeatedly passing the slide, film upwards, through a flame
until the glass is just uncomfortably hot when felt with the
back of the hand.
8.        Mark the slides with a grease pencil or fix labels on
them to indicate the nature of the smears.
9.        Place the slides back to back and wrap in a clean
paper.
10.        Enclose a brief description of the smears while
packing for despatch.

Pus:

Prepare a thin smear by spreading the pus evenly on a
side, with a sterile scalpel. In case this method fails to give
a satisfactory smear the following procedure may be
adopted:
1.        Place a small quantity of the pus on the middle of a
slide.
2.        Place a second slide on the first one so that the pus
is held between the two slides.
3.        Press gently the two slides together so as to spread
the pus.
4.        Hold the opposite ends of the two slides in each
hand and draw them apart, taking care not to lift one from
the other as this will cause the formation of lumps and
bubbles. If a thin smear is not obtained in the first attempt,
the slides may be placed together again and the process
repeated. 5. Dry the films by waving in the air, fix over a
flame, label, pack and despatch.

Secretions and excretions:

1.        These can be collected in clean vessels and sent
under refrigeration to the laboratory for detailed
examination. It is always better to have a styrofoam
(Thermocol) box filled with ice to carry the various
biological materials for culture and isolation of organism
and for sero-diagnosis for 3-5 ml of discharge.

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