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2.        The discharge can also be preserved using 10%
neutral formalin. 2 or 3 ml of this solution will be sufficient
for 3-5 ml of discharge.
3.        Smears can also be immediately prepared from the
discharges in clean slides and fixed rapidly by heat. These
should be wrapped properly and kept.
4.        For oozing lesions and cavities containing pus or
other materials (as often seen in TB of lungs) - a small
drop may be collected in a clean slide and uniformly spread
using a tooth pick or swab and fixed immediately in heat.
5.        In suspected cases of TB it is better to have material
scraped out from the lesions (after evacuating the
contaminated pus) collected on a clean slide and fixed.
Tissue
Tissue smears should be prepared as follows:
1.        Cut out a small piece of the organ, hold it in a pair of
forceps and rub its cut surface on the middle third of the
slide.
2.        If fluid accumulates on the cut surface, transfer a
very small quantity of the fluid to a slide with a sterile
scalpel and then spread it.
3.        If the cut surface is very dry, scrape it with a sterile
scalpel and spread the scraping evenly on a slide. This
may be done with the help of normal saline, if necessary.
4.        Caseating nodular and calcified lesions may be
treated in the manner described for pus.
5.        Fix and despatch as directed for blood smears.

BLOOD AND BLOOD SERUM

Blood Serum

1.        Select the appropriate vein of the ear and clean the
skin with anaesthetic ether or methylated spirit.

1.        Insert a sharp, large-bore, hypodermic needle into
the vein and collect the blood directly into a round,
screw-capped, wide-mouthed bottle of about 30-ml
capacity. The needle and the bottle should be perfectly
clean, sterile and dry. If on account of recent sterilisation,
the needle or the bottle shows some moisture on it, rinse it
thoroughly with physiological salt solution (0.9 g of sodium
chloride in 100 ml of boiled water) to prevent haemolysis. A
separate needle should preferably be used for each
animal. But if this is not possible, the needle should be
thoroughly washed out with the saline each time it is used
for bleeding.

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