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Materials for detection of poisons

•        Materials to be collected in suspected cases of
poisoning are as follows:
(a)        1000-1500 g of stomach contents and stomach
walls
(b)        1000-1500 g of intestinal contents
(c)        1000-1500 g of liver (d)1000g of spleen
(e)        Urine-1 litre in a separate bottle and put thymol as
a preservative
(f)        Kidney-one
•        In survival cases, the following materials may be sent
for analysis: stomach wash, stomach contents, vomitus,
blood, urine, faeces, water and feed.
•        Where the poison is suspected to be consumed by
inhalation, parts of small intestine with its contents, liver,
one kidney, lung, heart and brain tissues must also be
sent.
•        Uterus and foetus may also be useful in suspected
cases of abortion.
•        Burnt bones ashes should be preserved for analysis,
if dead body has been cremated. The skeleton or the
remnant bones are important materials for analysis in
cases of exhumed bodies where no visceral tissues are
available for toxicological examination.
Containers for preservation of materials
Wide-mouthed glass bottles of about 2-litre capacity
having airtight stoppers should be used for visceral
tissues. These bottles should be numbered and labelled
properly which should mention about the details of the
case, nature of the contents preserved, place and date of
preservation etc., and should bear the signature of the
veterinarian.
Preservation of tissues
The tissues are taken into the container and sufficient
alcohol is added so that whole tissues are dipped into the
solution.
Tissues are also preserved in saturated sodium chloride
solution (some excess quantity of undissolved salt should
remain at the bottom). Solid common salt may also be
used for preservation of the post-mortem tissues. The
tissues are taken into the container and sufficient quantity
of common salt is added to it. The tissues are immersed
well with the salt. Some salt should remain at the bottom of
the container and over the tissues.
A sample of alcohol or saturated solution of common salt
used for preservation must also be sent in separate glass
bottles for analysis to exclude the presence of any poison
in it.
Preservation of blood sample
The following preservation may be used for preservation of
blood sample:
1.        Sodium fluoride 20 mg/ml of blood
2.        Solution containing 10 g of sodium citrate and 200
mg of mercuric chloride dissolved in 100 ml of distilled
water. One drop of this solution is sufficient for each ml of
blood. The appropriate amount of fluid is taken into the
clean dry glass bottle; the fluid is dried so that the salt
remains sticking in the bottle. Then bottles are cooled at
room temperature and the sample of blood to be
preserved is taken into these bottles.

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